Descoberto mecanismo molecular que confere propriedades físicas especiais aos cromossomos na divisão de células humanas

Organização dos cromossomos mitóticos (magenta) e microtúbulos do fuso (verde) em uma fase inicial da divisão celular. Logo após o que é mostrado na imagem, os microtúbulos invadirão o espaço nuclear. No entanto, a compactação da cromatina regulada pela acetilação das histonas impedirá a perfuração dos cromossomos pelos microtúbulos. Crédito: ©Gerlich/IMBA

Como o genoma é empacotado em cromossomos que podem ser movidos fielmente durante a divisão celular.

Cientistas do Grupo Gerlich do IMBA – Instituto de Biotecnologia Molecular da Academia Austríaca de Ciências – descobriram um mecanismo molecular que confere propriedades físicas especiais aos cromossomos na divisão das células humanas para possibilitar seu transporte fiel à progênie. A equipe de pesquisa mostrou como uma modificação química estabelece um limite de superfície nítido nos cromossomos, permitindo que eles resistam à perfuração por microtúbulos do aparato do fuso. Suas descobertas são publicadas hoje (3 de agosto de 2022) na revista Natureza.

Quando as células se dividem, elas precisam transportar exatamente uma cópia do genoma para cada uma das duas células filhas. A segregação fiel do genoma requer o empacotamento de cromossomos extremamente longos[{” attribute=””>DNA molecules into discrete bodies so that they can be efficiently moved by the mitotic spindle, a filament system composed of thousands of microtubules. The new findings by the Gerlich Research Group at IMBA – Institute of Molecular Biotechnology of the Austrian Academy of Sciences – shed light on how mitotic chromosomes resist the constant pushing and pulling forces generated by the microtubules. “Amidst this complex system, the distinct physical properties are conferred to the chromosomes by changing the levels of histone acetylation, a chemical modification within the chromatin fiber,” says IMBA Group Leader Daniel Gerlich.

Prior work had shown that, in dividing cells, the chromatin fibers are folded into loops by a large protein complex called condensin. However, the role of condensin alone could not explain why chromosomes appear as dense bodies with a sharp surface rather than a loose structure resembling a bottlebrush. Some studies had suggested a role of histone acetylation in regulating the level of compaction during cell division, but the interplay of histone acetylation with condensin and its functional relevance remained unclear. “With our work, we are now able to conceptually disentangle the two mechanisms,” states Gerlich.

The team varied the levels of condensin and histone acetylation to study their precise effects. Removing condensin disrupted the elongated shape of chromosomes in dividing cells and lowered their resistance to pulling forces but did not affect their level of compaction. Combining condensin depletion with a treatment that increases the levels of histone acetylation caused massive chromatin decompaction in dividing cells, and perforation of chromosomes by microtubules.

The researchers hypothesized that chromatin is organized as a swollen gel throughout most of the cell cycle (when it is relatively highly acetylated) and that this gel compacts to an insoluble form during cell division when the acetylation levels globally decrease. They then developed an assay to probe the solubility of chromatin by fragmenting mitotic chromosomes into small pieces. The fragments of mitotic chromosomes formed droplets of liquid chromatin, but when the acetylation level was increased, the chromatin fragments dissolved in the cytoplasm. These observations support a model where a global reduction of chromatin acetylation during mitosis establishes an immiscible chromatin gel with a sharp phase boundary, providing a physical basis for resistance against microtubule perforation.

With further experiments involving pure chromatin that was reconstituted in vitro, and by probing chromatin access by various soluble macromolecules, the team found that immiscible chromatin forms a structure dense in negative charge that excludes negatively charged macromolecules and microtubules. “Our study shows how DNA looping by the condensin complex cooperates with a chromatin phase separation process to build mitotic chromosomes that resist both pulling and pushing forces exerted by the spindle. The deacetylation of histones during cell division hence confers unique physical properties to chromosomes that are required for their faithful segregation,” concludes Daniel Gerlich.

Reference: “A mitotic chromatin phase transition prevents perforation by microtubules” 3 August 2022, Nature.
DOI: 10.1038/s41586-022-05027-y

Funding: Austrian Science Fund, Vienna Science and Technology Fund, Vienna Science and Technology Fund, Howard Hughes Medical Institute, NIH/National Institutes of Health, Welch Foundation, Boehringer Ingelheim Fonds